ZFIN Image: Hsu et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Overexpression of rac2 rescues neutrophil recruitment in miR-722-expressing larvae. (A) Tg(lyzC:miR-722/Dendra2)^pu6 (miR-722) was crossed with Tg(mpx:mCherry-2A-Rac2) (Rac2) and the offspring were separated into four groups. For control, Tg(lyzC:miR-722/Dendra2)^pu6 was crossed with Tg(mpx:mCherry) (mCherry). All experiments were performed with F2 larvae at 3 dpf. (B) Tracks and quantification of neutrophil motility in indicated lines. Results were pooled from three independent larvae. P values were calculated with unpaired Student's t-test. Scale bar: 100 µm. (C,D) Quantification of neutrophil recruitment to tail wounding (C) or localized ear infection (D) in siblings separated into the four groups as depicted in A. One representative experiment of three independent biological repeats is shown (n=20 for each group). P values were calculated with unpaired two-way ANOVA.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.