FIGURE

Fig. S7

ID

ZDB-FIG-171229-25

Publication

Tarifeño-Saldivia et al., 2017 - Transcriptome analysis of pancreatic cells across distant species highlights novel important regulator genes

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Expression and function of myt1b in zebrafish pancreas. A: Whole-mount ISH showing expression pattern of myt1b in zebrafish embryos at 27 hpf. High expression is detected in the dorsal pancreatic bud (indicated by the arrow) and in the central nervous system. B: Quantification of glucagon and insulin expression at 48 hpf in wild-type (non-injected) embryos and in embryos injected with the 4 CRISPR myt1a/b guide RNA and Cas9. Graph B shows the volume of all gcga⁺ cells and all ins⁺ cells measured in each embryo by the imaging software Imaris (see Methods) (each point is the volume measured in one embryo). This quantification indicates a statistically significant reduction of the volume of alpha cell mass while beta cell mass is not drastically affected in the injected (F0) embryos (results of one experiment).

Expression Data

Gene:	myt1b
Fish:	WT
Anatomical Terms:	central nervous system posterior pancreatic bud
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-myt1a + CRISPR1-myt1b + CRISPR2-myt1a + CRISPR2-myt1b
Knockdown Reagents:	CRISPR1-myt1a CRISPR1-myt1b CRISPR2-myt1a CRISPR2-myt1b
Observed In:	pancreatic A cell pancreatic A cell differentiation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.