FIGURE

Fig. S1

ID

ZDB-FIG-171229-21

Publication

Tarifeño-Saldivia et al., 2017 - Transcriptome analysis of pancreatic cells across distant species highlights novel important regulator genes

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Expression of the transgenes Tg(ins:GFP), Tg(gcga:GFP), Tg(sst2:GFP), and Tg(ins:NTR:mCherry) in the endocrine pancreatic cell types. (A–C) Whole mount immunostaining of 3 dpf transgenic larvae Tg(ins:GFP) demonstrating the selective expression of GFP in beta cells. (D–I) Immunostaining of pancreas sections from adult transgenic fish Tg(gcga:GFP/ins:NTR:mCherry). Glucagon⁺ cells (see arrows in D–F) express high level of GFP and are not labelled by mCherry, while many insulin expressing cells are labelled by mCherry and by GFP (at slightly lower levels) (see arrowheads in G–I). These data reveal a leaking expression of the gcga:GFP transgene in beta cells. (J–L) ISH performed on pancreas section of adult Tg(sst2:gfp) with sst2 probe followed by immunofluorescence using the GFP antibody; the expression of endogenous sst2 gene co-localize with GFP staining confirming the specific expression of the transgene in delta cells [28].

Expression Data

Genes:	EGFP GFP mCherry sst2
Fish:	gz18Tg ulg021Tg ia1Tg; jh4Tg
Anatomical Terms:	pancreatic A cell pancreatic B cell pancreatic D cell
Stage Range:	Protruding-mouth to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.