P53 mediates the decrease of HSPCs in miR-142a-3p mutants. (a) P53 MO-injected 142T?/? embryos showed the restoration of runx1 expression at 26 and 36?hpf by WISH (left panel). The quantification of runx1-positive cells in the AGM was shown in the right panel (meanąs.d., n=3, *P<0.05). (b) qPCR showed that compared with 142T?/? at 36?hpf, the decrease in runx1 expression was rescued in 142T ?/? embryos injected with p53 MO (meanąs.d., n=3, *P<0.05). (c) WISH showed the expression of runx1 in 142T+/+ p53+/+, 142T?/? p53+/+ and 142T?/? p53?/? embryos at 36?hpf. There was a rescue effect on the decreased runx1 expression in 142T ?/? p53?/? double mutants. The quantification of runx1-positive cells in the AGM was shown in the right panel (meanąs.d., n=60, *P<0.05).
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