FIGURE

Fig. 3

ID

ZDB-FIG-171129-16

Publication

Sharma et al., 2016 - ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions

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Fig. 3

Zebrafish pronephric structure and function are adversely affected by hyperglycaemia and upon CRISPR mediated ELMO1 knockout.

As compared to the zebrafish pronephros at 48 hpf in control morphants, Co. Mo., (A) hyperglycaemic PDX1 morphants, PDX1 Mo., (B) display an enlarged glomerulus (white arrow head) and highly shortened pronephric neck (white asterix). (C) Control crispanst (Co. CRISPR) display a normal glomerulus. (D) In contrast, ELMO1 crispants, (ELMO1 CRISPR), show an enlarged glomerulus (white arrow head) and a highly shortened pronephric neck (white asterix). (E–H) The altered structure in morphants and crispants has been quantified in n = 40 embryos for each condition. (I) Displays the quantified elevated loss of fluorescence in ELMO1 crispants (n = 38) and PDX1 morphants (n = 26) as compared to their respective controls (Co. CRISPR, n = 39 and Co. Mo., n = 30). The white line in image (D) represents the scale bar corresponding to 200 μm.

Expression Data

Gene:	EGFP
Fish:	li1Tg li1Tg + CRISPR1-elmo1 li1Tg + MO1-pdx1
Knockdown Reagents:	CRISPR1-elmo1 MO1-pdx1
Anatomical Terms:	pronephric glomerulus pronephric tubule
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg + CRISPR1-elmo1 li1Tg + MO1-pdx1
Knockdown Reagents:	CRISPR1-elmo1 MO1-pdx1
Observed In:	pronephric glomerulus pronephric tubule pronephros morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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