FIGURE

Fig. 6

ID

ZDB-FIG-171127-68

Publication

Lin et al., 2016 - Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae

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Fig. 6

Deficiency of stmn4 prolonged the G2 phase in a Cdc25a-dependent manner.

(a) 1-cell stage embryos were injected without (Ctrl) or with 5 and 10 ng stmn4 tMO₂. A 15-min BrdU pulse (green arrowhead) was then given to embryos at 16 hpf. Part of embryos were fixed at an hour intervals from 17–19 hpf and subjected to double immunochemistry against α-BrdU (green) and α-pH3 (red). Fixed embryos were examined and photographed under confocal microscopy. Representative photos are presented for each treatment at designated times post the BrdU pulse. All images are shown in dorsal views with anterior to the top. Percentages of pH3+ and BrdU+ cells (yellow cells) within the pH3+ cells (yellow and red cells) were calculated as shown in (b) and analyzed by one-way ANOVA. (c) 1-cell stage Tg(Huc:Kaede) embryos were injected without or with 5 ng stmn4 tMO₂ and indicated amounts of cdc25a plasmid. The percentages of embryos with premature elavl3 expression were examined and presented as described in Fig. 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-stmn4
Knockdown Reagent:	MO2-stmn4
Observed In:	cell population proliferation mitotic G2 phase
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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