FIGURE

Fig. 4

ID

ZDB-FIG-171127-66

Publication

Lin et al., 2016 - Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae

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Fig. 4

Overexpression of CA-Stmn4 or depletion of stmn4 by shRNA induced elavl3 precocious expression in dorsal midbrain.

(a) Conditional gene expression system was introduced by combining Gal4-UAS system with heat shock 70 (hsp70) promoter. After heat shock, upstream activation sequence (UAS) elements will be recognized by Gal4 protein dimers (indicated by light blue) and then activate transcription of downstream genes including nuclear H2A-mCherry in red and Stmn4 intervening with a viral 2A peptide. (b) Flow chart showing the conditional expression system. We heat-shocked the Tg(HuC:Kaede) embryos injected with both plasmids described above until bud stage. After two hours of induction, embryos were analyzed until 16 hpf for elavl3 expression revealed by Kaede (green). (c) Dorsal view of midbrains between epiphysis (indicated by white arrows) and (TgSNs, circled by green dashed lines) are shown. Within one embryo, average number of precocious elavl3 (demonstrated by Kaede, green) expressing cells colocalized with (yellow) or without overexpressions of stmn4 either with mutations or its corresponding shRNA^stmn4_614 (indicated by mCherry, red) in dorsal midbrain, are shown on the left-bottom corner of each panel. Scale bars are 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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