FIGURE

Fig. 5

ID

ZDB-FIG-171127-28

Publication

Ota et al., 2016 - Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish

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Fig. 5

Functional analysis of Tg[epdr1-hs:eGFP].

(a) Autofluorescence of epidermis in wild-type embryo (red asterisk). (b,c) The distribution of eGFP-positive cells in the midbrain (mb) and the hindbrain (hb) of heterozygous (b) and homozygous (c) Tg[epdr1-hs:eGFP] embryos at 30 hpf (lateral view, anterior left) (confocal images). Autofluorescence in epidermis is indicated by red asterisk. (d) The area of eGFP-positive cells of heterozygous and homozygous Tg[epdr1-hs:eGFP] embryos in the MHB (red dashed line) and anterior part of hindbrain (blue dashed line) in the visual field (N = 14 each). Error bars indicate standard error of the mean (SEM). Statistical significance was determined using Student’s t-test. *P < 0.05.

Expression Data

Gene:	EGFP
Fish:	epdr1^{uy220Tg/uy220Tg} epdr1^uy220Tg/+
Anatomical Terms:	hindbrain midbrain hindbrain boundary
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	epdr1^{uy220Tg/uy220Tg}
Observed In:	midbrain hindbrain boundary
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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