FIGURE

Fig. 3

ID

ZDB-FIG-171114-19

Publication

Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis

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Fig. 3

Loss of syn4 increased neuroglial cells.

(A–R'') WISH results of gfap (A–F''), slc1a3a (G–L'') and olig2 (M–R'') at 24 hpf and 48 hpf in zebrafish. (D–F') The expression of gfap, (J–L'') slc1a3a, (Q–R'') olig2 are higher in the syn4 morphants than controls at 48 hpf. (S) sqRT-PCR analyzes of the transcript level of gfap, slc1a3a and olig2. (T–W) Densitometric quantification of sqRT-PCR. The syn4 morphants shown a higher expression level of gfap, slc1a3a and olig2 (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). FV: ventricular zone in forebrain, Hb: hindbrain, PM: posterior midbrain, sc: spinal cord. Lateral views, dorsal to the top and anterior to the left in (A–C,D–F,G–I,J–L',M–O,P–R,P''–R''), Dorsal view, anterior to the left in (D'–F',G'–I',J''–L'',P'–R').

Expression Data

Genes:	gfap olig2 slc1a3a
Fish:	AB AB + MO3-sdc4
Knockdown Reagent:	MO3-sdc4
Anatomical Terms:	astrocyte forebrain ventricular zone midbrain ventricular zone oligodendrocyte radial glial cell spinal cord spinal cord dorsal region whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-sdc4
Knockdown Reagent:	MO3-sdc4
Observed In:	forebrain ventricular zone midbrain ventricular zone spinal cord spinal cord dorsal region whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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