FIGURE

Fig. 4

ID

ZDB-FIG-171113-32

Publication

Powell et al., 2016 - Zebrafish M�ller glia-derived progenitors are multipotent, exhibit proliferative biases and regenerate excess neurons

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Fig. 4

The ultimate localization of MG-derived progenitors becomes progressively more biased at later rounds of proliferation.

Fish were given an intraperitoneal injection of BrdU at 2 dpi, followed by an injection of EdU at 3, 6, or 12 dpi. Each sample was harvested at 14dpi. (A?C) EdU+ nuclei were counted at the times indicated and the percentage of EdU+ nuclei residing in the (A) ONL, (B) INL and (C) GCL was determined for each injury model. Data represents means?�?s.d. (n???3). *P?<?0.02941. (D,E) The percentage of ONL EdU+ nuclei residing in (D) the upper region or (E) the lower region was determined for each injury model. (F) Co-staining samples for BrdU and EdU demonstrates that the cells proliferating at later times are a subpopulation of those proliferating at earlier times. (G?L) Representative images of retinal sections analyzed in (A?F) that were stained for EdU at (G?I) 3 dpi or (J?L) 6 dpi following (G,J) needle poke, (H,K) PA or (I?L) NMDA injury. Scale bar is equal to 50??m. Abbreviations are as in Fig. 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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