Fig. 6

Knockdown of mog1 causes a decrease of expression levels of cardiac transcriptional factors in the anterior lateral mesoderm by whole-mount in situ hybridization.

(A–D) Knockdown of mog1 expression reduces intensity of the nkx2.5 signal. Zebrafish embryos (1–2 cell stage) were injected with 16 ng of Std-MO (A,C), 16 ng of mog1 MO1 (B) or 16 ng of mog1 MO2 (D) and used for whole-mount in situ hybridization at 12 hpf with an antisense probe for nkx2.5. (G–J) Knockdown of mog1 expression reduces intensity of the gata4 signal. The embryos were injected and processed as in (A–D), but probed with an antisense probe for gata4. (M–P) Knockdown of mog1 expression reduces expression of hand2. (S–V) Knockdown of mog1 expression does not affect expression of gata5. (E,F,K,L,Q,R,W,X) Over-expression of zebrafish mog1 did not affect expression of cardiac transcriptional factors in the anterior lateral mesoderm at the 12 hpf stage. (A–R1,S–X) Embryos are shown at a dorsal view with the anterior at the top. (M2–R2) embryos are shown in a lateral view with the anterior on the left. The numbers in the upper right corner (e.g. 27/31 in A) describe the ratio of the number of embryos with the phenotype (e.g. 27) in the image over the number of total embryos analyzed (e.g. 31). Scale bar = 100 μM.