FIGURE

Fig. 1

ID

ZDB-FIG-171009-27

Publication

Soman et al., 2017 - Inhibition of the mitochondrial calcium uniporter rescues dopaminergic neurons in pink1^-/- zebrafish.

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Fig. 1

mcu expression. (A) RT-PCR analysis showing consistent expression of mcu throughout development (1, 24, 48 and 72 hpf). (B) In situ hybridization with dorsal (left) and lateral (right) views of mcu expression in 3 dpf zebrafish larvae, demonstrating strong expression in the brain. (C) Quantification of mcu mRNA at 3 dpf, demonstrating similar expression levels in wt and pink1^−/−. (D) RT-PCR analysis of mcu knockdown using antisense morpholino (MO) at 3 dpf, demonstrating the marked effect of MO mediated mcu k/d on mcu wild type transcript levels. (E) Q-PCR based confirmation of marked effect of MO-mediated mcu k/d on mcu mRNA transcript levels (****P < 0.0001).

Expression Data

Gene:	mcu
Fish:	WT pink1^sh397/sh397 WT + MO3-mcu
Knockdown Reagent:	MO3-mcu
Anatomical Terms:	brain whole organism
Stage Range:	4-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-mcu
Knockdown Reagent:	MO3-mcu
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Neurosci.