FIGURE

Fig. S2

ID

ZDB-FIG-170829-7

Publication

Abrial et al., 2017 - TGF-β Signaling Is Necessary and Sufficient for Pharyngeal Arch Artery Angioblast Formation

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Inhibition of TGFβ signaling does not grossly disrupt the body or cranial vasculature, Related to Figure 1.

(A-D) Lateral views of 36 hpf Tg(kdrl:GFP) embryos treated from 20 hpf with DMSO (A,B) or 50μM LY-364947 (C,D) imaged in the green channel on a compound microscope to visualize endothelium (pseudocolored magenta). The body vasculature appeared similar between LY-364947-treated and DMSOtreated embryos. Magnified views (B,D) of boxed regions (A,C) highlight intersomitic vessels. (E-J) Left lateral views of 36 hpf Tg(kdrl:GFP), Tg(gata1:DsRed) embryos treated from 20 hpf with DMSO (E-G) or 50μM LY-364947 (H-J) imaged on a confocal microscrope in the green and red channels to visualize endothelium (pseudocolored magenta) and red blood cells (RBC) (pseudocolored yellow), respectively. Merged (E,H) and single channel (F,G,I,J) images are shown. In both cohorts, cranial vascular development and integrity appeared grossly normal and supported RBC circulation. (A,C) Scale bars = 200μm, (B,D-J) Scale bars = 100μm. (n=4 to 5 embryos per group)

Expression Data

Genes:	DsRed GFP
Fish:	la116Tg la116Tg; sd2Tg
Condition:	chemical treatment by environment: LY 364947
Anatomical Terms:	nucleate erythrocyte vascular endothelium
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.