Fig. S4

Sox sites ablation compromises notch1b-15 enhancer activity. (A) A representative confocal projection of wild-type zebrafish F₀ larvae injected with notch1b-15:GFP (top panel) and notch1b-15:mutSOX-a/b:GFP (bottom panel). Bright GFP expression could be observed in the intersomittic vessel and dorsal aorta of fish injected with the wild-type notch1b-15 construct, whereas expression of GFP in fish injected with the mutant construct is almost completely absent from the arterial vascular network (white arrows). (B) Graph indicating levels of GFP expression in F₀ zebrafish transgenic for notch1b-15:GFP and notch1b-15mutSOX-a/b:GFP transgenes. All scored fish were also positive for the internal control cardiac actin:RFP (+RFP). Number of zebrafish examined for each conditions was indicated, expression was scored as no (-), weak or strong GFP . (C) Scatter plots representing the relationship between genomic GFP copy number and GFP intensity in endothelial lining of dorsal aorta (left panel) and intersomittic vessels (right panel) in stable tg(notch1b-15) and tg(notch1b-15mutSOX-a/b:GFP). Only larvae with GFP copy number <10 were included in the analysis for Fig. 7B-C. Scored tg(notch1b-15), n=46; tg(notch1b-15mutSOX-a/b:GFP), n=42; larvae were each pooled from 4 independent founders.