FIGURE

Fig. S6

ID

ZDB-FIG-170809-17

Publication

Mesureur et al., 2017 - Macrophages, but not neutrophils, are critical for proliferation of Burkholderia cenocepacia and ensuing host-damaging inflammation

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Fig. S6

Related to Figs 6 and 7. Macrophages, but not neutrophils, contribute to increased bacterial burden and pro-inflammatory responses towards subcutaneous B. cenocepacia.

(A) mpeg/umn⁺ embryo infected subcutaneously at 2 dpf with B. cenocepacia K56-2 (green). Confocal stack (59 slices, 1 μm) at 24 hpi showing bacteria in macrophages. A close up of the indicated area is shown in 3D, with volume rendering for GFP signal and surface rendering of mCherry signal without (middle panel) and with 50% surface transparency (right panel). Scale bar 50 μm. (B) Fluorescence images showing non-infected mpx/umn⁺ and mpeg1/umn⁺ control embryos at 3 dpf, the time point that resembles 24 h post subcutaneous injection in Fig 6. (C) Image overlay (bright field, red and blue filters) of the tail region of an mpx/umn⁺ embryo after subcutaneous injection with B. cenocepacia K56-2 expressing Turquoise, presenting tissue damage by 24 hpi. Scale bar, 100 μm. (D) Image overlay (bright field, red, blue and green filters) of the tail region of an Mtz-treated mpeg1/umn⁺; mpx:GFP embryo 24 h after subcutaneous injection with B. cenocepacia K56-2 expressing Turquoise. Scale bar, 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.