ZFIN Image: Mesureur et al., 2017, Fig. S6

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Figure Caption

Fig. S6

Related to Figs 6 and 7. Macrophages, but not neutrophils, contribute to increased bacterial burden and pro-inflammatory responses towards subcutaneous B. cenocepacia.

(A) mpeg/umn⁺ embryo infected subcutaneously at 2 dpf with B. cenocepacia K56-2 (green). Confocal stack (59 slices, 1 μm) at 24 hpi showing bacteria in macrophages. A close up of the indicated area is shown in 3D, with volume rendering for GFP signal and surface rendering of mCherry signal without (middle panel) and with 50% surface transparency (right panel). Scale bar 50 μm. (B) Fluorescence images showing non-infected mpx/umn⁺ and mpeg1/umn⁺ control embryos at 3 dpf, the time point that resembles 24 h post subcutaneous injection in Fig 6. (C) Image overlay (bright field, red and blue filters) of the tail region of an mpx/umn⁺ embryo after subcutaneous injection with B. cenocepacia K56-2 expressing Turquoise, presenting tissue damage by 24 hpi. Scale bar, 100 μm. (D) Image overlay (bright field, red, blue and green filters) of the tail region of an Mtz-treated mpeg1/umn⁺; mpx:GFP embryo 24 h after subcutaneous injection with B. cenocepacia K56-2 expressing Turquoise. Scale bar, 100 μm.

Acknowledgments

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