FIGURE

Fig. 1

ID

ZDB-FIG-170807-9

Publication

Tao et al., 2017 - The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma

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Fig. 1

Loss of function of def affects sympathetic neuronal development in zebrafish. (a–j) Whole-mount in situ hybridization for HuC (a and b), sox10 (c and d), phox2b (e and f), th (g and h) and dβh (i and j) in def^−/− mutant and sibling fish at the indicated genotypes and time points. (k–m) SCG signal intensity indicated by th (g and h), dβh (i and j) or EGFP (n–q) in fish of different def backgrounds was calculated. Horizontal bars indicate the means±s.d., which were compared with the two-tailed unpaired t-test. ***P<0.001; ****P<0.0001. (n–q) Schematic (n) and representative images (o–q) of SCG (arrows) development at 5 dpf in TgEGFP transgenic line with wild-type def (TgEGFP), def^+/− (def^+/−;TgEGFP) or def^−/− (def^−/−; TgEGFP) background. DA, diencephalic dopaminergic neurons; dpf, days post fertilization; LC, locus coeruleus; ns, not significant; R, retina; SCG, superior cervical ganglion.

Expression Data

Genes:	dbh EGFP elavl3 phox2bb sox10 th
Fish:	AB utp25^{hi429Tg/hi429Tg} (AB) zdf15Tg (AB) utp25^hi429Tg/+; zdf15Tg (AB) utp25^{hi429Tg/hi429Tg}; zdf15Tg (AB)
Anatomical Terms:	superior cervical ganglion whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	utp25^{hi429Tg/hi429Tg} (AB) utp25^{hi429Tg/hi429Tg}; zdf15Tg (AB)
Observed In:	neural crest cell development superior cervical ganglion sympathetic nervous system development
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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