FIGURE

Fig. 6

ID

ZDB-FIG-170620-16

Publication

Erickson et al., 2017 - Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt)

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Fig. 6

Restoration of MET channel activity in mercury mutant hair cells with heat shock-inducible Tomt-GFP.

(A) Workflow for the heat-shock inducible tomt-GFP transgene experiment. (B) Representative images of Tomt-GFP (left panels), FM 4–64 (middle panels) and merged GFP, FM 4–64 and DIC channels (right panels) in lateral line NMs of 5 dpf Tg(hsp70l:tomt-GFP); tomt^nl16 larvae pre- and post-heat shock. (C, D) Quantification of GFP intensity (C) and FM 4–64 intensity (D) per NM of Tg(hsp70l:tomt-GFP); tomt^nl16 larvae (n = 6) pre- and post-heat shock. Error bars are the mean ± SD. Asterisks indicate p<0.0001 by unpaired, two-tailed t-test. Scale bars: 5 µm in B.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tomt^nl16/nl16; vo20Tg
Condition:	heat shock
Observed In:	neuromast hair cell stereocilium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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