FIGURE

Fig. 2

ID

ZDB-FIG-170620-12

Publication

Erickson et al., 2017 - Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt)

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Fig. 2

Tomt protein localization.

(A) Diagram of the protein made from the Tg(myo6b:tomt-GFP) transgene plus an image of Tomt-GFP localization in live hair cells of the anterior crista in the inner ear at 3 dpf. The arrow points to an apical hair bundle. (B) Diagram of the protein made from the Tg(myo6b:tomt-HA) transgene plus immunolabel for the HA epitope showing Tomt-HA localization in the hair cells of the anterior crista in the inner ear at 4 dpf. Phalloidin stain (magenta) marks F-Actin. (C) Co-localization between Tomt-GFP and a medial Golgi marker (Mgat1a_1–110-mKate2) in live hair cells of the lateral crista (3 dpf). The hair bundle region is indicated by the white bracket. (D) Diagram of the protein made from the Tg(myo6b:tomt_1–45-GFP) transgene plus an image of Tomt_1–45-GFP localization in live hair cells of the lateral crista at 4 dpf. Scale bars: 5 µm in A-D.

Expression Data

Genes:	EGFP mKate2
Fish:	vo31Tg vo24Tg; vo31Tg
Anatomical Terms:	anterior crista hair cell hair cell Golgi apparatus lateral crista hair cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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