FIGURE

Fig. 4

ID

ZDB-FIG-170517-37

Publication

Anelli et al., 2017 - Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Analysis of a cohort of transgenic zebrafish expressing BRAF^V600E in thyrocytes at 5 months post fertilization.

(A–B) Photographs and epifluorescent photographs of live five mpf tg-TOM (A) and Tg-BRAF^V600E-TOM fish (B) reveals TdTomato-positive tissue. (C) Representative H&E and IHC using anti-thyroglobulin antibody of sagittal section of adult fish tg-TOM at five mpf. (D) Representative images from three tg-BRAF^V600E-TOM adult fish at five mpf demonstrating the presence of invasive carcinoma. Top panel, mass of Tg-positive thyroid cells (arrows) invading muscle. Middle panel, thyroid cells invading gill tissue (arrows), inset is 40X. Lower panel, thyroid cells invading subcutaneous fat in the ventral jaw (arrows), inset is 40X. (E) Western blot analysis of thyroid obtained from two tg-TOM (control) and four tumors bearing tg-BRAF^V600E-TOM zebrafish. Western blot analysis was performed in duplicate, a representative blot is shown. (F) Quantitative analysis of fold change in pERK/total ERK from (E), p<0.05, Student’s t-test.

Expression Data

Gene:	Tomato
Antibodies:	Ab1-tg Ab9-mapk Ab12-mapk
Fish:	wcm102Tg wcm101Tg
Anatomical Term:	thyroid follicle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wcm101Tg
Observed In:	thyroid follicle
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife