Fig. 6

Notch inhibition phenocopies the sox10baz1 DRG phenotype.

A-H) Posterior trunks of WT embryos treated with 100 μM DAPT from 30–72 hpf (C,D,G,H) and controls exposed to DMSO carrier alone (A,B,E,F). E-F show enlargements of A-D. Treated embryos show dorsally-curved body axis (C). Immunofluorescence for Elav1/Hu revealed increased numbers of DRG sensory neurons (arrowhead). I,J) Quantitation of DRG sensory neurons in 5 dpf embryos after treatment with 100 μM DAPT (I, orange) or 100 μM Compound E (J, orange) from 30–72 hpf; compared with control DMSO-treated embryos (yellow in I and J), sensory neuron numbers are significantly increased (***) when Notch signaling is inhibited. While trunk refers to the entire somitic region from posterior to the otic vesicle to the anus, tail refers to the post-anal body; posterior trunk refers to the 7 most posterior somatic segments dorsal to the yolk sac extension. N = 13 (Control, I), 13 (DAPT, I), 16 (Control DMSO, J) and 15 (Comp. E, J). Significance of elevated numbers of neurons was evaluated by two-tailed Student’s t test (**, p<0.01; ***, p<0.001).