Fig. S7
Estimation of Embryo Center Position and Measurement of EVL Surface Projections, Related to STAR Methods (A and B) Estimation of embryo center position. Side views of the animal pole hemisphere were obtained from two perpendicular directions in the two-photon microscope stack as maximum intensity projections (A). A circle was fitted along the arc of this hemisphere projection (red dotted line), and the center of the fitted circle was used to estimate the embryo center (B). (C-K) Image processing of EVL surface projections. Z-stack images obtained by two-photon microscopy were first processed by binarization (C and D). The outline of each image slice was detected and its size was reduced by the expected thickness of surface cells (E and F). The detected and reduced outline was applied to the original image slice, and the inner area of this outline was filled with black color (G and H). After processing the entire stack with this method, they were superimposed by maximum intensity projection (I). The resultant projection image shows clearer surface cell outlines compared to a projection image from an unprocessed stack. (J-K'). White rectangle regions in (J) and (K) are magnified in (J') and (K'), respectively. |
Reprinted from Developmental Cell, 40(4), Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P., The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation, 354-366.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell