PUBLICATION

The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation

Authors
Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P.
ID
ZDB-PUB-170222-18
Date
2017
Source
Developmental Cell   40(4): 354-366.e4 (Journal)
Registered Authors
Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Morita, Hitoshi
Keywords
active fluid description of tissue spreading, gastrulation, interfacial tension, radial cell intercalation, surface cell expansion, zebrafish
MeSH Terms
  • Animals
  • Biophysical Phenomena*
  • Blastoderm/cytology
  • Blastoderm/metabolism
  • Cell Communication
  • Cell Count
  • Cell Movement
  • Cell Proliferation
  • Computer Simulation
  • Embryo, Nonmammalian/cytology
  • Gastrulation*
  • Morphogenesis*
  • Stress, Physiological
  • Surface Tension
  • Zebrafish/embryology*
  • Zebrafish/physiology*
PubMed
28216382 Full text @ Dev. Cell
Abstract
Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during "doming," when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.
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