FIGURE

Fig. 4

ID

ZDB-FIG-170308-5

Publication

Bower et al., 2017 - Vegfd modulates both angiogenesis and lymphangiogenesis during zebrafish embryonic development

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Fig. 4

Vegfd mRNA injection induces artery defects in a Kdr-dependent manner. (A-F) Injection of vegfd RNA into Tg(fli1a:egfp) embryos causes bilateral turning of aISVs (arrows) at 28-32 hpf (n=31, A,B), which is rescued by co-injection of a morpholino against kdr (C,D), but not flt4 morpholino injection (E,F). (G,H) Co-injection of flt1 morpholino and vegfd RNA increases the bilateral turning of aISVs (n=20) when compared with vegfd RNA (n=31) or flt1 morpholino alone (n=29) (compare A,B,G and H). (I,J) Quantification of experiments presented in A-E (I) and G,H (J). Data represent mean±s.e.m.; ****P<0.0001, ***P<0.001 from one-way ANOVA. Data were obtained from three independent injections. (K) qPCR analysis of vegf receptor and ligand expression in uninjected and vegfd mRNA-injected embryos shows no significant difference in mRNA levels for any of the genes examined.

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO1-kdr y1Tg + MO3-flt1 y1Tg + MO3-flt4
Knockdown Reagents:	MO1-kdr MO3-flt1 MO3-flt4
Anatomical Term:	trunk vasculature
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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