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Fig. 4
Vegfd mRNA injection induces artery defects in a Kdr-dependent manner. (A-F) Injection of vegfd RNA into Tg(fli1a:egfp) embryos causes bilateral turning of aISVs (arrows) at 28-32 hpf (n=31, A,B), which is rescued by co-injection of a morpholino against kdr (C,D), but not flt4 morpholino injection (E,F). (G,H) Co-injection of flt1 morpholino and vegfd RNA increases the bilateral turning of aISVs (n=20) when compared with vegfd RNA (n=31) or flt1 morpholino alone (n=29) (compare A,B,G and H). (I,J) Quantification of experiments presented in A-E (I) and G,H (J). Data represent mean±s.e.m.; ****P<0.0001, ***P<0.001 from one-way ANOVA. Data were obtained from three independent injections. (K) qPCR analysis of vegf receptor and ligand expression in uninjected and vegfd mRNA-injected embryos shows no significant difference in mRNA levels for any of the genes examined.