FIGURE

Fig. 5

ID

ZDB-FIG-170303-17

Publication

Kuri et al., 2017 - A high-sensitivity, bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real-time

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Fig. 5

Specificity of Tg(8xHs.NFκB:GFP,Luciferase) for NF-κB activity in zebrafish embryos. (A,B) 0.3 mM iκbαa morpholino was injected into transgenic embryos. Morphants at 3 dpf had increased expression of GFP in the skin (A) and proctodeum (B, arrows). GFP fluorescence is shown as a false-color heat map. The intensity of the laser was equal for both images to allow direct comparison of the GFP signal in morphants and controls. (C) Quantitative comparison of the GFP signal in the proctodeum of embryos injected with 0.3 mM standard control or iκbαa morpholino. Each dot represents an individual embryo at 2 dpf (mean±s.d.). (D) Injection of 1 mM iκbαa morpholino results in higher GFP expression and embryonic malformation. GFP fluorescence is shown as a heat map as indicated in A. Scale bars: 500 µm (A); 50 µm (B); 300 µm (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.