FIGURE

Fig. 6

ID

ZDB-FIG-170227-16

Publication

De Angelis et al., 2017 - Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover

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Fig. 6

ISV Sprouting Can Be Restored by Derepressing Vegf Signaling or Treating with HA'se/o-HA

(A) Experimental design to examine the effect of repressing and derepressing Vegf signaling on ISV sprouting using the inhibitor, SU5416.

(B) Lateral view images of Tg(kdrl:mCherry) embryos treated according to scheme in (A). Scale bar, 25 μm.

(C) Scatterplots showing SU5416 treatment from 16 somites to 48 hpf causes complete failure of ISV formation (n = 10) compared with normal sprouting after similar DMSO treatment (n = 13). By contrast, removal of SU5416 at 30 hpf, after ISV sprouting has failed, restores ISV sprouting (n = 10). Addition of SU5416 from 30 hpf had no significant effect on ISV number (n = 10).

(D and E) Lateral view images showing the phenotype of sibling or tmem2^hu5935 mutant embryos at (D) 28 hpf pre-injection and (E) 42 hpf following HA'se or o-HA injection. Scale bar, 100 μm.

(F) Scatterplot demonstrating no difference in number of ISVs in sibling embryos injected with dextran (n = 11), HA'se (n = 10), or o-HA (n = 8) at 28 hpf and measured at 42 hpf; however, tmem2^hu5935 mutant embryos have significant restoration of ISV number following injection of HA'se (n = 9) or o-HA (n = 10) compared with dextran control (n = 20). ^∗∗∗∗p < 0.0001. Error bars represent ±SD.

Expression Data

Gene:	EGFP
Fish:	s843Tg cemip2^{hu5935/hu5935}; s843Tg
Condition:	chemical treatment by environment: semaxanib
Anatomical Terms:	intersegmental vessel trunk vasculature
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg cemip2^{hu5935/hu5935}; s843Tg
Condition:	chemical treatment by environment: semaxanib
Observed In:	intersegmental vessel
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 40, De Angelis, J.E., Lagendijk, A.K., Chen, H., Tromp, A., Bower, N.I., Tunny, K.A., Brooks, A.J., Bakkers, J., Francois, M., Yap, A.S., Simons, C., Wicking, C., Hogan, B.M., Smith, K.A., Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover, 123-136, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell