FIGURE

Fig. 4

ID

ZDB-FIG-170227-14

Publication

De Angelis et al., 2017 - Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover

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Fig. 4

Depolymerized HA Rescues the tmem2 Mutant Phenotype

(A) Experimental design for hyaluronidase (HA'se) or o-HA injection.

(B) Lateral views of entire trunks of sibling and tmem2^hu5935 mutant embryos post-injection and imaged at 38–42 hpf. Yellow asterisks indicate the absence of sprouts in uninjected mutants. Representative images demonstrate phenotypic rescue of tmem2^hu5935 mutants following injection with HA'se or o-HA. Scale bar, 150 μm.

(C) Scatterplots summarizing unaffected siblings in the uninjected (n = 23), HA'se injected (n = 23), o-HA injected (n = 13) or hyaluronidase heat-inactivated (HA'se inact) (n = 26) embryos.

(D) Uninjected tmem2^hu5935 mutants (n = 19) have significantly fewer ISVs that uninjected siblings. Mutants injected with HA'se (n = 17) or o-HA (n = 14) have significantly rescued phenotypes compared with uninjected mutants. Heat-inactivated HA'se injected mutants were not significantly different from uninjected mutants. ^∗∗∗∗p < 0.0001; ns, not significant. Error bars represent ±SEM.

Expression Data

Gene:	EGFP
Fish:	y1Tg cemip2^{hu5935/hu5935}; y1Tg
Anatomical Term:	trunk vasculature
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cemip2^{hu5935/hu5935}; y1Tg
Observed In:	intersegmental vessel
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 40, De Angelis, J.E., Lagendijk, A.K., Chen, H., Tromp, A., Bower, N.I., Tunny, K.A., Brooks, A.J., Bakkers, J., Francois, M., Yap, A.S., Simons, C., Wicking, C., Hogan, B.M., Smith, K.A., Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover, 123-136, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell