FIGURE

Fig. 8

ID

ZDB-FIG-170224-14

Publication

Hamm et al., 2016 - Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1

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Fig. 8

Sema3d-Nrp1 signaling regulates cell–cell contacts and Actin cable formation in the CCV leading edge by activating RhoA. (A and B) Lateral confocal projections of Tg(fli1a:lifeactEGFP)mu240 embryos at 30 hpf. Magnifications of the leading edges were color-inverted. (A) sema3d morphants and mutants as well as nrp1a/b morphants exhibited lesions in the leading edge (pink arrowheads) and lacked parallel-arranged Actin cables (open arrowhead in Ctr MO). (B) Activation of RhoA from 24 to 30 hpf reduced lesions (pink arrowheads) and rescued Actin cable formation (open arrowheads) in sema3d morphants and mutants as well as in nrp1a/b morphants. (C and D) Quantification of cell–cell contact length (C, n = 16) and cells with Actin cables (D, each n = 8; sema3d^mu268 + RhoA activator, n = 4). ***, P < 0.001; **, P < 0.01. Error bars indicate SD.

Expression Data

Gene:	EGFP
Fish:	mu240Tg mu240Tg + MO2-nrp1a mu240Tg + MO2-sema3d sema3d^mu268/mu268; mu240Tg
Knockdown Reagents:	MO2-nrp1a MO2-sema3d
Anatomical Term:	common cardinal vein blood vessel endothelium
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mu240Tg + MO2-nrp1a mu240Tg + MO2-sema3d sema3d^mu268/mu268; mu240Tg
Knockdown Reagents:	MO2-nrp1a MO2-sema3d
Observed In:	common cardinal vein actin filament bundle common cardinal vein actin filament bundle organization common cardinal vein blood vessel endothelial cell common cardinal vein cell-cell junction venous blood vessel morphogenesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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