FIGURE
Fig. 1
- ID
- ZDB-FIG-170222-53
- Publication
- Lebedeva et al., 2017 - Characterization of genetic loss-of-function of Fus in zebrafish
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Fig. 1
Generation and validation of the fus knockout zebrafish. (A) fus alleles generated by CRISPR-Cas9. A screenshot from the UCSC genome browser shows aligned sequences from heterozygous F1 animals. CRISPR target site in fus exon 3 is underlined in red. (B) An example of a PAGE gel for genotyping the Δ8 allele. M = Low molecular weight marker. Wild type (75bp) and fus−/− (67bp) products can be discriminated. (C) Quantification of fus mRNA relative to WT mRNA in 5dpf embryos. (D) Normal morphology of the WT and fus−/− embryo at 6 d post fertilization (dpf). Scale bar is 1mm. |
Expression Data
| Gene: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | Day 5 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Observed In: | |
| Stage Range: | Day 5 to Day 6 |
Phenotype Detail
Acknowledgments
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