FIGURE

Fig. S4

ID

ZDB-FIG-170220-42

Publication

Wild et al., 2017 - Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization

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Fig. S4

Flt1 targeting morpholinos and quantification of endothelial cell numbers

(a) Heart rates in 4dpf WT and flt1^ka601, mean ± s.e.m, n=10. (b-e) flt1^ka601 with 1ng (d) and 3ng (e) flt1 targeting MO injected. Note: the 3ng dosage causes arterial branching defects (arrowheads) not observed in flt1^ka601 mutants. (f,g) Vascular pattern in Tg(kdrl:EGFP) embryos injected with control MO (f), and 1ng flt1 ATG targeting MO (g). (h) Quantification of f,g; mean ± s.e.m, n=10. Note hyper-branching equal to flt1ka601. (i-k) Imaging of endothelial nuclei in 17hpf WT and flt1^ka601 embryos. EC numbers were counted between somite 7-9 (i). (l) Quantification of j,k shows no difference in EC numbers. ECs were counted using ImageJ plugin 3D object counter, mean ± s.e.m, n=4. MO, morpholino. Scale bar, 50μm in b-g; 10μm in j,k.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.