FIGURE

Fig. 1

ID

ZDB-FIG-170220-31

Publication

Wild et al., 2017 - Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization

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Fig. 1

Expression of Vegf receptors and ligands at the neurovascular interface.

(a,b) Double transgenic embryos TgBAC(flt1:YFP)^hu4624; Tg(kdrl:hsa.HRAS-mcherry)⁹¹⁶ at 30 hpf and 3 dpf shows flt1 expression (green) in dorsal aorta, arterial ISV and dorsal aspect of venous ISV (3 dpf) and neurons (arrowheads). (c) 3D-rendered view of vessels (green) and nerves (purple) in Tg(kdrl:EGFP)^s843;Tg(XIa.Tubb:DsRed)^zf148 embryos highlighting dorsal aspect of ISVs (arrowheads) in close contact to the neural tube (NT). (d) Transverse section of the trunk of TgBAC(flt1:YFP)^hu4624;Tg(XIa.Tubb:DsRed)^zf148 embryos shows that ISVs (green, arrowhead) and neural tube (NT, red) are in close contact. Dorsal is up. (e) Magnified view of purple-boxed area in (d), showing direct contact of vessels with nerves at the neurovascular interface (blue arrowheads) and flt1 expressing neurons with long axonal extensions in the neural tube (white arrowheads). (f) Magnified view of blue-boxed area in (d) showing flt1 expressing neurons (arrowheads) and their axons inside neural tube (red). (g) Lateral view of TgBAC(flt1:YFP)^hu4624; Tg(Xla.Tubb:DsRed)^zf148 at the level of the neural tube showing flt1 expressing neurons (arrowheads) in neural tube. DA, dorsal aorta; dpf, days post fertilization; DLAV, dorsal longitudinal anastomotic vessel; hpf, hours post fertilization; ISV, intersegmental vessel; NC, notochord; NT, neural tube; PCV, posterior cardinal vein. Scale bar, 30 μm in a–d,g; 10 μm in e,f.

Expression Data

Genes:	DsRed EGFP mCherry YFP
Fish:	hu4624Tg; zf148Tg s843Tg; zf148Tg hu4624Tg; s916Tg
Anatomical Terms:	blood vessel dorsal aorta dorsal longitudinal anastomotic vessel intersegmental artery intersegmental vein intersegmental vein dorsal region intersegmental vessel posterior cardinal vein spinal cord spinal cord neuron
Stage Range:	Prim-15 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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