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Fig. 4

ID

ZDB-FIG-170123-27

Publication

Drummond et al., 2017 - The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish

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Fig. 4

tbx2a/b deficiency leads to an increase in the number of cells in the CS. (A) The CS was labeled by whole mount in situ hybridization to detect transcripts encoding stc1 at the 28 ss stage. (B) In wild-type embryos, each CS is typically comprised of about 4–6 stc1⁺ cells per nephron. However, tbx2a/b deficiency in morphants and fby^c144 mutants induced a significant increase of the stc1⁺ cell number in the CS. (C) Labeling the CS with sim1a revealed a similar phenotype consistent with an inhibitory role of tbx2a/b in CS development. (D) Quantification of sim1a+ CS cell number revealed that tbx2a, tbx2b and tbx2a/b morphants and fby^c144 mutants all led to a significantly greater number of CS cells than wild-type embryos. For each experiment, at least 15 morphant embryos and 3 genotypically confirmed fby^c144 mutant embryos were examined. (*p<0.05, **p<0.001). Abbreviations: CS (corpuscle of Stannius).

Expression Data

Genes:	sim1a stc1
Fish:	TU tbx2b^c144/c144 TU + MO4-tbx2a TU + MO4-tbx2b TU + MO1-tbx2
Knockdown Reagents:	MO1-tbx2 MO4-tbx2a MO4-tbx2b
Anatomical Term:	corpuscles of Stannius
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx2b^c144/c144 TU + MO4-tbx2a TU + MO4-tbx2b TU + MO1-tbx2
Knockdown Reagents:	MO1-tbx2 MO4-tbx2a MO4-tbx2b
Observed In:	corpuscles of Stannius corpuscles of Stannius cell
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.