Fig. S1

glypican4^fr6 primordia have morphology defects and deposit fewer neuromasts. (A-B) Compared to control siblings where the primordium has an elongated ovoid shape (A), glypican4^fr6 mutant primordia are more rounded and of smaller size (B). (C) Cell counts by DAPI staining show no significant differences between sibling and glypican4^fr6 mutant primordia at 24 hpf (p-value (t-test)=0.327). (D) The reduced size of the 24 hpf glypican4^fr6 mutant primordia is not due to reduced proliferation (p-value (t-test)=0.682). (E-F) Bright field images of a sibling control and a glypican4^fr6 mutant embryo at 34 hpf depicting the embryo trunk phenotypes. (E′-F′) Same embryos represented in (E) and (F) under fluorescent light showing the lateral line primordium migrating along the trunk. No-turning glypican4^fr6 mutant primordia reach the tail tip shortly after 34 hpf, whereas the sibling primordium has just passed the yolk extension. (G-J) Lateral views of 34 hpf embryos depicting representative phenotypes of sibling (G) and the three phenotypes of glypican4^fr6 mutant primordia migration (H-J). (K-N) Lateral views of 56 hpf embryos depicting representative phenotypes of sibling (K) and the three phenotypes of glypican4^fr6 mutant primordia migration (l-N). (O) Quantification of deposited neuromasts at 58 hpf in sibling versus glypican4^fr6 mutants (siblings=5.61±0.08 vs. glypican4^fr6(total)=1.98±0.17 neuromasts, n=56 embryos. sibling vs. glypican4^fr6 (total) p-value=1.7×10⁻³⁶; sibling vs. glypican4^fr6 (No-turn) p-value=2.38×10⁻³⁵; sibling vs. glypican4^fr6 (U-turn) p-value=1.77×10⁻³³). Error bars in (C), (D) and (O) represent standard errors and (*) represent statistically significantly phenotypes.