FIGURE

Fig. 1

ID

ZDB-FIG-161221-48

Publication

Tornini et al., 2016 - Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration

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Fig. 1

tph1b Regulatory Sequences Permit Clonal Analysis of Blastemal Cells

(A and B) tph1b:mCherry reporter expression in regenerating fins at 2 (A) and 5 (B) days post-amputation (dpa).

(D) Design of lineage tracing experiments in (E and F).

(E and F) tph1b:CreER; ubi:switch fins were treated with 4 μM tamoxifen from 24–36 hr post-amputation (hpa) and imaged at 5 (E) and 20 (F) dpa.

(G) Section through 3-dpa lineage-labeled tph1b:CreER; ubi:switch fin, stained for osteoblasts (green) using the Zns5 antibody as well as nuclei (DAPI, blue). e, epidermis; o, osteoblasts, if, intraray fibroblasts.

(H) Design of clonal analysis experiments in (I–K).

(I) Representative image of a single labeled blastemal cell (arrow) in a 2-dpa blastema.

(J) Two-dimensional projection of all initial labeling events onto a rescaled virtual blastema, with rescaled lateral coordinates (D, dorsal; V, ventral) and initial PD coordinates (amputation plane, P, proximal; distal tip, D, distal), color coded by time of detection of the initial labeling event.

(K) Two-dimensional projection of all initial labeling events onto a rescaled virtual blastema, color coded by ray of origin (1 indicates lateral-most ray, 8 indicates the medial ray). Dashed lines, amputation planes.