FIGURE

Fig. 1

ID

ZDB-FIG-161027-36

Publication

Middel et al., 2016 - Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair

Other Figures

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Fig. 1

Macrophages remove repair patch.

(a-c) Repair patch (AnxA2a-mO, a, white arrow) and macrophages (mpeg1:GFP, b, white arrows) before (Initial), 240 s and 1,500 s after wounding. A macrophage ingests parts of repair patch (green arrows). (c) Merged views of a,b. Note the bleed-through of AnxA2a-mO into the GFP channel (b). (d) Repair patch removal in control (left, n=37) and macrophage-depleted embryos (KD, right, n=25, Fisher’s exact test P<10^-8) over 20 h. (e) Crispr/Cas9 knock-out of irf8 impairs repair patch removal. In controls, the repair patch was removed in 32% of injured myofibers (n=19) within one hour after injury. In the irf8-KO embryos the patch was present in all cases of injured myofibers examined (n=15). This suggests that morpholino triple knock-down is inefficient and the same effect on macrophages can be achieved by elimination of irf8 at the stage analysed. Significance was checked with Fishers exact test at P<0.05 (P=0.023895). Scale bar, 4 µm.

Expression Data

Gene:	EGFP
Fish:	gl22Tg
Condition:	light damage: sarcolemma: muscle cell
Anatomical Term:	macrophage
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	irf8^ka90/ka90; gl22Tg gl22Tg + MO1-irf8 + MO1-spi1b + MO3-csf3r
Condition:	light damage: sarcolemma: muscle cell
Knockdown Reagents:	MO1-irf8 MO1-spi1b MO3-csf3r
Observed In:	macrophage muscle cell cell wall repair
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.