FIGURE

Fig. S1

ID

ZDB-FIG-161024-1

Publication

Nissim et al., 2016 - Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development

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Fig. S1

Nr5a2 is required for the formation of the mature pancreas and liver. A) nr5a2 expression in zygote through early segmentation stages. nr5a2 is maternally deposited and expressed in the dividing blastomeres. nr5a2 mRNA is actively shuttled to the dividing blastomeres in vesicles (black arrows) by the 1- cell to 2- cell stage. Expression of nr5a2 persists to approximately 12 hpf, after which it is temporarily inactivated. B) nr5a2 morpholino injected Tg(fabp10a:DsRed) embryos have a reduced embryonic liver at 80 hpf. C) nr5a2^oz3/oz3 embryos lack a mature exocrine pancreas by in situ hybridization for carboxypeptidase A5 (carbA). D) trypsin+ acinar cells fail to form in the pancreas region (blue arrows) of nr5a2^oz3/oz3 mutant embryos by 56 hpf.

Expression Data

Genes:	cpa5 DsRed nr5a2 prss1
Fish:	WT nr5a2^oz3/oz3 nr5a2^oz3/+ gz4Tg gz4Tg + MO2-nr5a2
Knockdown Reagent:	MO2-nr5a2
Anatomical Terms:	acinar cell blastomere exocrine pancreas liver whole organism yolk vesicle
Stage Range:	2-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nr5a2^oz3/oz3 gz4Tg + MO2-nr5a2
Knockdown Reagent:	MO2-nr5a2
Observed In:	acinar cell exocrine pancreas liver
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.