ZFIN Image: Nissim et al., 2016, Fig. S1

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Fig. S1

Nr5a2 is required for the formation of the mature pancreas and liver. A) nr5a2 expression in zygote through early segmentation stages. nr5a2 is maternally deposited and expressed in the dividing blastomeres. nr5a2 mRNA is actively shuttled to the dividing blastomeres in vesicles (black arrows) by the 1- cell to 2- cell stage. Expression of nr5a2 persists to approximately 12 hpf, after which it is temporarily inactivated. B) nr5a2 morpholino injected Tg(fabp10a:DsRed) embryos have a reduced embryonic liver at 80 hpf. C) nr5a2^oz3/oz3 embryos lack a mature exocrine pancreas by in situ hybridization for carboxypeptidase A5 (carbA). D) trypsin+ acinar cells fail to form in the pancreas region (blue arrows) of nr5a2^oz3/oz3 mutant embryos by 56 hpf.

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Acknowledgments

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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.