FIGURE

Fig. S4

ID

ZDB-FIG-160926-38

Publication

Bhuiyan et al., 2016 - Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

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Fig. S4

(A) Relative gene expression of IL-8 and Lta4H in wild-type A. baumannii (ATCC 17978) and ΔgacS-infected zebrafish embryos at 1 and 12 hpi. Compared with uninfected embryos, mRNA for IL-8 and Lta4H was increased, but there was no significant difference between wild-type A. baumannii and ΔgacS-infected embryos. Experiments were performed at least in triplicate with three biological replicates (mean ± SEM). (B) Neutrophil clustering was not seen for an A. baumannii mutant involving another GacS-regulated gene (csuD) in a somatic muscle (red rectangle) (i) or otic vesicle (red circle) (ii) infection at 48 hpi. (C) Neutrophil chemotaxis was seen, but dwelling was not observed for heat-killed A. baumannii ΔpaaA (HK ΔpaaA) infection at 48 hpi. (Magnification: B and C, 10×.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA