FIGURE

Fig. S1

ID

ZDB-FIG-160926-33

Publication

Bhuiyan et al., 2016 - Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

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Fig. S1

Zebrafish survival after bloodstream infection with clinical A. baumannii strains: (A) AB307, (B) A9844, (C) AB0059, and (D) ATCC 19606 (n = 30 embryos, three biological replicates; P value is a comparison of 3 × 10² and 1 × 10² cfu per embryo by log-rank test). (E) Bacterial density over time in zebrafish. At a lower infection inoculum, zebrafish were able to control and clear infection [median (range) of five homogenized embryos at each time point, pooled data from four biological replicates and compared using unpaired t test with Welch’s correction]. (F) When zebrafish were infected into the yolk sac, hypersusceptibility to lethal infection was observed (n = 30 embryos, three biological replicates; P value is a comparison of 1.6 × 10² and 1 × 10³ cfu per embryo by log-rank test). (G) Histopathological section taken at 2 h through a localized somatic muscle infection with A. baumannii-GFP. Neutrophils are red fluorescent [Tg(lyz:DsRed)^nz50] with DAP counterstain (white arrow indicates phagocytosed bacteria). (Scale bar: 40 µm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA