FIGURE

Fig. 7

ID

ZDB-FIG-160805-3

Publication

Spicer et al., 2016 - Targeted Mutagenesis of the Hypophysiotropic Gnrh3 in Zebrafish (Danio rerio) Reveals No Effects on Reproductive Performance

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Fig. 7

gnrh3^-/- juveniles exhibit normal Gnrh3 neuronal migration.

In both gnrh3^+/+ gnrh3:tdTomato (B) and gnrh3^-/- gnrh3:tdTomato (C) fish, Gnrh3-tdTomato-expressing soma located in the olfactory region and ventral telencephalon project fibers that extend posteriorly toward the hypothalamus and pituitary stalk (pituitaries not shown; A). Z-stack images were taken at 20x magnification on a Leica Microsystems DMi8 confocal microscope with a resolution of 1024 x 1024 and a z-step size of 0.10. All images were analyzed and assembled with Image J and Adobe Photoshop. Scale bars = 100 µm. OB = olfactory bulb. T = telencephalon. OT = optic tectum. ON = optic nerves. POA = pre-optic area. P = pituitary.

Expression Data

Gene:	Tomato
Fish:	mb102Tg gnrh3^zf713/zf713; mb102Tg
Anatomical Terms:	hypophysis cell projection membrane olfactory bulb cell body olfactory bulb cell projection membrane ventral telencephalon cell body ventral telencephalon cell projection membrane
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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