ZFIN Image: Spicer et al., 2016, Fig. 7

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Figure Caption

Fig. 7

gnrh3^-/- juveniles exhibit normal Gnrh3 neuronal migration.

In both gnrh3^+/+ gnrh3:tdTomato (B) and gnrh3^-/- gnrh3:tdTomato (C) fish, Gnrh3-tdTomato-expressing soma located in the olfactory region and ventral telencephalon project fibers that extend posteriorly toward the hypothalamus and pituitary stalk (pituitaries not shown; A). Z-stack images were taken at 20x magnification on a Leica Microsystems DMi8 confocal microscope with a resolution of 1024 x 1024 and a z-step size of 0.10. All images were analyzed and assembled with Image J and Adobe Photoshop. Scale bars = 100 µm. OB = olfactory bulb. T = telencephalon. OT = optic tectum. ON = optic nerves. POA = pre-optic area. P = pituitary.

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Acknowledgments

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