FIGURE

Fig. 2

ID

ZDB-FIG-160706-8

Publication

Raman et al., 2016 - aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis

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Fig. 2

The levels of apical Lgl increase in the apical domain of the head peridermal cells in has/apkc mutants.Immunostaining using anti-Lgl2 and E-cadherin antibodies (a) in wild-type and has/apkc mutant embryos. Immuno-colocalization of Lgl and F-actin in the apical domain of wild-type and has/apkc mutant embryos at 48 hpf (b). Colocalization coefficient analysis between Lgl and F-actin by Pearson’s (PC) method and Manders’ overlap for Lgl with F-actin (M1) and F-actin with Lgl (M2) at 48 hpf in wild-type and has/apkc mutant embryos (c). Asterisks in c indicate significant difference at P<0.001 by Student’s t-test. Scale bar in a and b is equivalent to 10 µm.

Expression Data

Antibodies:	Ab1-llgl2 Ab2-cdh1
Fish:	WT prkci^m567/m567
Anatomical Terms:	peridermal cell actin-based cell projection peridermal cell apical side
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prkci^m567/m567
Observed In:	peridermal cell actin-based cell projection peridermal cell apical side
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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