FIGURE

Fig. 5

ID

ZDB-FIG-160705-1

Publication

Rupprecht et al., 2016 - Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy

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Fig. 5

Calcium imaging in the olfactory bulb using configuration 1. (a) Calcium indicator fluorescence (512 x 512 pixels) in four optical sections through the OB of an adult Tg(NeuroD:GcaMP6F)_icm05 fish (see Visualization 1). Values of d show the displacement of the center of each image from the reference plane (no mirror displacement; D = 0 and d = 0). Raw fluorescence images were averaged over the full 200 s. Two glomerular structures are outlined in black. Right: scan trajectory in yz; both scalebars are 50 µm. (b,c) Relative change in fluorescence intensity during application of each odor (40 s). (d) Time courses of first three principal components (PC 1-3) (e) Correlation maps showing the correlation coefficient between the time course of each pixel and each PC. For each optical section, the correlation maps for each PC are combined into a single RGB image (see color cube).

Expression Data

Gene:	GCaMP
Fish:	icm05Tg
Anatomical Term:	olfactory bulb
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express