ZFIN Image: Rupprecht et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Calcium imaging in the olfactory bulb using configuration 1. (a) Calcium indicator fluorescence (512 x 512 pixels) in four optical sections through the OB of an adult Tg(NeuroD:GcaMP6F)_icm05 fish (see Visualization 1). Values of d show the displacement of the center of each image from the reference plane (no mirror displacement; D = 0 and d = 0). Raw fluorescence images were averaged over the full 200 s. Two glomerular structures are outlined in black. Right: scan trajectory in yz; both scalebars are 50 µm. (b,c) Relative change in fluorescence intensity during application of each odor (40 s). (d) Time courses of first three principal components (PC 1-3) (e) Correlation maps showing the correlation coefficient between the time course of each pixel and each PC. For each optical section, the correlation maps for each PC are combined into a single RGB image (see color cube).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express