FIGURE

Fig. S4

ID

ZDB-FIG-160630-36

Publication

Lovely et al., 2016 - Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish

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Fig. S4

Blocking Bmp signaling using a hs:dnBmpR1a-GFP transgenic line results in similar defects as Dorsomorphin treatment. (A&B) Whole-mount images of the viscerocranium of 5 dpf embryos labeled with Alcian Blue and Alizarin Red. (A) Control, heat-shocked embryos do not display any craniofacial defects. (B) The hs:dnBmpR1a-GFP embryos, heat-shocked at 10 hpf have severe craniofacial defects. (C&D) Images of heat-shocked sox17:dsred transgenic embryos. (C′-D′′) Imaris surface renderings of images in C&D, with C′′&D′′ being rotated 45° into the plane of view. (E&F) lateral views of sox10:mRFP. Embryos expressing hs:dnBmpR1a-GFP had defects to both endoderm and pharyngeal arch morphology. (A&B) ventral views, (C-F) lateral views, anterior to the left. Scale bar (A, B) = 100 µm, (C-F) = 50 µm, Mc = Meckel’s cartilage, Ch = ceratohyal cartilage, P = pouches PA = pharyngeal arches.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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