FIGURE

Fig. 2

ID

ZDB-FIG-160608-17

Publication

Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish

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Fig. 2

The non-HSC tissues are unaffected in the cyp2aa9 morphants.

(a-d) The cyp2aa9 morphants in bright-field were morphologically normal (a, n = 110/110; b, n = 125/130) and with intact vasculature at 33 hpf under the Tg(kdrl:GFP) background (c, n = 21/21; d, n = 21/25). (e-t) The cyp2aa9 morphants displayed normal expressions of the following tissue-specific markers: primitive haematopoiesis (etsrp, e,f; scl, g,h; gata1, i,j), notochord (shh, k,l), dorsal aorta (flt1, m,n; ephrinB2, o,p) and posterior cardinal vein (flt4, q,r; dab2, s,t). (a-d,i-t) Lateral views, anterior left, dorsal up. (e-h) Dorsal views, anterior left.

Expression Data

Genes:	dab2 efnb2a etsrp flt1 flt4 gata1a shha tal1
Fish:	WT WT + MO1-cyp2aa3,cyp2aa9
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Anatomical Terms:	dorsal aorta hematopoietic multipotent progenitor cell notochord posterior cardinal vein
Stage Range:	5-9 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cyp2aa3,cyp2aa9 s843Tg + MO1-cyp2aa3,cyp2aa9
Knockdown Reagent:	MO1-cyp2aa3,cyp2aa9
Observed In:	vasculature whole organism
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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