FIGURE

Fig. 6

ID

ZDB-FIG-160524-6

Publication

Eno et al., 2016 - aura/mid1ip1L regulates the cytoskeleton at the zebrafish egg-to-embryo transition

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Fig. 6

Distribution of exocytic and endocytic factors in aura mutants. (A,B) The exocytic factor Rab11 properly localizes to the tips of microtubules at the furrow in wild-type (5/5) and aura mutant (6/6) embryos. (C,D) The exocytic factor Vamp2 localizes to mature furrows in wild type (5/5) but not in mutants (7/7). (E-H) The endocytic factors Clathrin and Dynamin 2 become enriched in ectopic CGs in aura mutants (F,F′,H,H′), whereas they exhibit a disperse cortical distribution in wild type (E,E′,G,G′). Clathrin appears localized to the CG surface in ectopic CGs in aura mutants (5/5 embryos, compared with 10/10 wild-type embryos with diffuse cortical labeling). Dynamin 2 also localizes to ectopic CGs (15/15 embryos, compared with 13/13 wild-type embryos with diffuse cortical labeling), which can appear as ring-like structures (3/15 embryos as shown in H′) or throughout the granule surface (12/15 embryos as shown in Fig. S5M,M′). Ectopic localization to CGs was confirmed by colabeling with WGA (not shown). (A′-H′) Higher magnifications of boxed regions in A-H. Scale bars: 100µm in A-H; 10µm in A′-H′.

Expression Data

Antibodies:	Ab1-dnm2 Ab1-rab11ba Ab1-vamp2 Ab2-cltc
Fish:	AB mid1ip1l^t9792/t9792
Anatomical Terms:	blastomere blastomere cleavage furrow blastomere cortical granule blastomere microtubule
Stage Range:	1-cell to 4-cell

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mid1ip1l^t9792/t9792
Observed In:	blastomere cleavage furrow
Stage:	4-cell

Phenotype Detail

Acknowledgments

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