FIGURE

Fig. 4

ID

ZDB-FIG-160524-4

Publication

Eno et al., 2016 - aura/mid1ip1L regulates the cytoskeleton at the zebrafish egg-to-embryo transition

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Fig. 4

aura mutants retain CGs. (A,B) During egg activation (2mpf), CGs appear embedded in an F-actin network in both wild type (A) and aura mutants (B). (C,D) By 10mpf, wild-type eggs have extruded nearly all CGs (C), whereas a large fraction of CGs are retained in aura mutants (D) (wild type, 0 mpf n=152, 10 mpf n=3; mutant, 0 mpf n=245, 10 mpf n=130; chi-square, P<0.0001). By 10mpf, the cortical F-actin cytoskeleton appears as a network of short F-actin fibers in aura mutants (D′′), in contrast to being largely disassembled as in wild type (C′′). (A′-D′) Higher magnification views of A-D; the boxed regions are further magnified in A′′-D′′. Scale bars: 100µm in A-D; 10µm in A′-D′,A′′-D′′.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mid1ip1l^t9792/t9792
Observed In:	cortical granule exocytosis early embryonic cell cortical granule
Stage:	1-cell

Phenotype Detail

Acknowledgments

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