FIGURE

Fig. 1

ID

ZDB-FIG-160524-45

Publication

Kague et al., 2016 - Osterix/sp7 limits cranial bone initiation sites and is required for formation of sutures

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Fig. 1

Zebrafish sp7 mutants have delayed early bone development. (A) Sp7 protein sequences of human, mouse, medaka and zebrafish were aligned using ClustalW. Identical residues are shaded dark gray. Cysteine and histidine residues corresponding to the zinc finger domains from positions 318 to 402 (black line) are highly conserved in a domain showing only three amino acid variations between mammals and fish. The mutation p.Leu145* (black triangle) introduces a stop codon, deleting over half of the Sp7 coding sequence, including the three zinc finger domains. (B and C). Calcified bones were detected at 4 dpf by vital Alizarin red staining. While WT (B) and mutant (C) larvae were indistinguishable by morphology, the cleithrum (arrows) and opercle (asterisks) are slightly smaller in sp7 mutants. In situ hybridization was performed for markers of osteoblast differentiation in embryos at 4 dpf. Expression of all markers in the cleithrum was reduced in mutants, especially spp1 and col10a1.

Expression Data

Genes:	col1a1a col10a1a spp1
Fish:	WT sp7^{hu2790/hu2790}
Anatomical Term:	cleithrum
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sp7^{hu2790/hu2790}
Observed In:	bone development bone element cleithrum opercle
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 413(2), Kague, E., Roy, P., Asselin, G., Hu, G., Stanley, A., Albertson, C., Simonet, J., Fisher, S., Osterix/sp7 limits cranial bone initiation sites and is required for formation of sutures, 160-72, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.